ECO:0000086

amplification of intermethylated sites evidence

Term id:ECO:0000086
Term name:amplification of intermethylated sites evidence
Definition:A type of in vitro methylation assay evidence resulting from a genome-wide estimate of DNA methylation by differential enzymatic digestion of genomic DNA with methylation-sensitive and methylation-insensitive isoschizomers, followed by restrained PCR amplification of sequences methylated at both ends and resolving the PCR products in a denaturing polyacrylamide-sequencing gel to generate fingerprints that consist of multiple anonymous bands that represent the DNA methylome of the cell. [PMID:18987810, url:https:www.ncbi.nlm.nih.govprobedocstechpcr]
Comment:The bands can be individually isolated and characterized which leads to the identification of hypo- and hypermethylation events.
Is a:ECO:0001065 ! in vitro methylation assay evidence
Synonym:AIMS EXACT []
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Is obsolete:False