ECO:0000109

reverse transcription polymerase chain reaction evidence

Term id:ECO:0000109
Term name:reverse transcription polymerase chain reaction evidence
Definition:A type of RNA detection assay evidence where an RNA transcript is reverse transcribed into cDNA and amplified to qualitatively detect gene expression. [ECO:SW, PMID:11013345, PMID:12901609]
Comment:The starting product for PCR, and therefore amplification volume, is directly correlated to the transcription rate.
Is a:ECO:0005518 ! RNA detection assay evidence
Synonym:reverse transcription polymerase chain reaction transcription evidence BROAD []; RT-PCR EXACT []; transcript levels (e.g. RT-PCR) RELATED []
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Alternate id:ECO:0000108
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Property value:IAO:0000112 Taken together, the results of the RT-PCR analyses and the reporter fusion assays indicate that expression of SMU.1882 is up regulated in the presence of CovR. xsd:string {xref=PMID:21124877}; IAO:0000112 The RT-PCR assay indicated that the sycO, ypkA and yopJ genes (designated as pCD12, pCD13 and pCD14 in Y. pestis 91001 [19], respectively) were transcribed as a single primary RNA (Fig. 1), and thereby these three genes constituted a single operon in Y. pestis Microtus strain 201. xsd:string {xref=PMID:19703315}; IAO:0000112 Using reverse transcription-PCR, we found that primers designed to span the intergenic regions between zur and the znuC homolog, as well as znuC and znuB homolog each generated a PCR product (Figure 2A). This indicated that these genes were transcribed on the same mRNA. xsd:string {xref=PMID:24086521}; IAO:0000234 CollecTF xsd:string
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Is obsolete:False